Webb1 juli 2006 · Ten 96-well microplates (Nunc Maxisorb; SLS, Nottingham, UK) were coated with each of the different antibodies (raised against human AMH, also reacting with rat AMH) at 2 μg/ml in 0.05 m bicarbonate buffer (pH 9.4) and incubated at 4 C overnight, before incubation with a blocking buffer containing 0.5% (wt/vol) casein (Mast Group … Webb21 jan. 2024 · Sections of 10–12 µm were rehydrated in PBS at 37 °C and then left for 5 min in hematoxylin, 1 min in alcoholic acid and 5 min in eosin. Sections were then rehydrated (70%, 90% 100% EtOH, each phase for 1 min) and mounted with DPX after having immersed them in Histoclear (National Diagnostics, SLS, Nottingham, UK).
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