Phis1525

Author: zfeb

August undefined, 2024

WebbAmplikon diinsersikan pada plasmid pHIS1525, yang keduanya telah didigesti dengan enzim restriksi Sac I dan Sph I. Plasmid rekombinan yang dihasilkan ditransformasikan … Webb巨大芽孢杆菌低温保存法：1.简单保存法，将琼脂斜面孢子培养物、菌丝悬浮液以及由麸皮、大米、小米等谷物原料制成的孢子培养物置于4℃冰箱保存,保存时间不超过1～2个月，若将谷物原料制备的孢子瓶抽真空并在棉塞上浸蜡,以隔绝外界空气和水汽,保持时间可达3～4个 …

Evaluation of promoter sequences for the secretory ... - Springer

Webb4 feb. 2012 · The aim of this research was to express the β-xylosidase gene in the pHIS1525/ Bacillus megaterium MS941 system. The xyl gene was amplified from pTP510 and cloned into pHIS1525 in E. coli DH10β. The recombinant plasmid was transformed into B. megaterium MS941 by protoplast transformation. Transformants were selected by … Webbb-Xylosidase encoding gene from G. thermoleovorans IT-08 had been expressed in the pHIS1525/ B. megaterium MS941 system. The b-xylosidase gene (xyl) was... DOAJ is a … portalkhecham

芽孢里面有dna吗_芽孢里面有dna吗商家/厂家/公司/_丁香通

WebbIn order to develop a stable strain of Bacillus megaterium for Keratinase production, the Keratinase gene (KerA) of Bacillus lichiniformis ATCC 53757 and SPlipA gene from plasmid pHIS1525.SPlipA (B ... WebbThe recombinant Bacillus megaterium MS941 expressed and secreted the β-xilosidase into culture medium 5 hours after adding 5% xylose. The b-xylosidase activity was 0.441 … Webb21 mars 2016 · pHIS1525 Like pMM1525 with additional sequence for C-terminal 6xHis-tag fusion (including stop codon). pSTREP1525 Like pMM1525 with additional sequence for … irvin house

Phis1525

Webb1 feb. 2006 · The vectors pHIS1525 and pSTREP1525 allow the insertion of target genes for the production and secretion of a C-terminally His 6-tagged and an N-terminally Strep II-tagged fusion protein, respectively (see the supplemental material). Webb1 Rekombinasi Gen Penyandi -xilosidase asal Geobacillus Thermoleovorans IT-08 dalam Plasmid Phis1525 (Recombinant Gen of Encoding -Xylosidase from Geo... Author: Siska …

Did you know?

WebbThe recombination plasmid pHIS1525 was transformed into Escherichia coli DH5a and the transformant was designated DH5a-pHIS1525-G7. The plasmid from the recombinant … Webb1 juli 2024 · Dengan demikian, baik amplikon gen maupun vektor kloning pHIS1525 mempunyai ujung-ujung lengket yang saling komplementer. Kesimpulan Dari hasil …

WebbDownload dengan 2 jenis enzim restriksi SacI dan SphI, (2) rekombinasi. (ligasi) amplikon dan plasmid pHIS1525 menggunakan enzim ligase (3) transformasi plasmid ... Webb巨大芽孢杆菌系统：phis1525 巨大宿主菌：yybm1，wh320 乳酸球菌表达系统 pmg36e,nz9000,mg1363 乳酸酵母表达系统 pklac1，gg799 白色念珠菌系统pcr4,pcr4-gfp,p5921 酿酒酵母表面展示系统：pyd1,eby100 标准微生物菌株 atcc27853 铜绿假单胞菌 atcc29212 粪链球菌 atcc29213 金黄色葡萄球菌

Webb431-1418-1-PB - Free download as PDF File (.pdf), Text File (.txt) or read online for free. Webb4 dec. 2009 · ISBN: 978-981-4322-10-2 (hardcover) USD 199.00. ISBN: 978-981-4464-19-2 (ebook) USD 80.00. Description. Chapters. This book aims to disseminate the most current research in applied microbiology presented at the III International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2009) held in Lisbon, …

http://www.biofeng.com/zaiti/qita/pHIS1525.html

WebbThis research showed that the b-xylosidase gene was succesfully sub-cloned in pHIS1525 system and expressed by the recombinant B. megaterium MS941. Theaddition of 0.5% … irvin house letchworthWebbTools. Data Hub. Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. Basic analysis for a user … irvin internationalWebbpHIS1525 Description: Plasmid type: Expression vector of Bacillus megaterium Size: 7402 bp Tags: 6xHis Resistance(s): Ampicillin (Amp) Selectable markers: Tetracycline (Tet) irvin hunt funeral homeWebbsubsequently cloned into Bacillus megaterium expression vector; pHIS1525.SP lipA and transformed in Bacillus megaterium ATCC®14945. The kerA gene carrying the … portallenpels twitterWebb4 feb. 2012 · The aim of this research was to express the β-xylosidase gene in the pHIS1525/ Bacillus megaterium MS941 system. The xyl gene was amplified from … portalite softboxWebbvector pHis1525 19. FliCD was expressed in B. megaterium and puried as described previously 20. Preparation of C. difﬁcile spores Sporulation of the C. difﬁcile R20291 strains were induced in Clospore medium as described previously 21. Brieﬂ y, an overnight 20 ml of C. difﬁcile cultured in Columbia Broth was inoculated into 500 ml of irvin international incWebbThe aim of this research was to express the Î²-xylosidase gene in the pHIS1525/ Bacillus megaterium MS941 system. The xyl genewas amplified from pTP510 and... DOAJ is a … irvin hoover prints